BBa_K395164
BBa_K395164 Version 1 (Component)
KanR activated by lasR and 3OC12HSL(AHL of Las family)

BBa_K329057
BBa_K329057 Version 1 (Component)
[KanR + LacZ] in between MUT_MUT[HK022] Tn916 Arms

BBa_K329056
BBa_K329056 Version 1 (Component)
[KanR + LacZ] in between WT_MUT[HK022] Tn916 Arms

BBa_K1969007
BBa_K1969007 Version 1 (Component)
Nano-lantern(cAMP-1.6) regulated by ADH1 promoter

BBa_K1969013
BBa_K1969013 Version 1 (Component)
Nano-lantern(cAMP-1.6) regulated by GAL1 promoter

BBa_K329055
BBa_K329055 Version 1 (Component)
[KanR + LacZ] in between MUT_MUT[Lambda] Tn916 Arms

BBa_K329054
BBa_K329054 Version 1 (Component)
[KanR + LacZ] in between WT_MUT[Lambda] Tn916 Arms

Kan-BA-Bbs
BBa_K349010 Version 1 (Component)
KanR-BA-BbsI (for construction of BA BioBytes 2.0)

KanR-AB-Bf
BBa_K349011 Version 1 (Component)
KanR-AB-BfuAI (for construction of AB BioBytes 2.0)

KanR-AB-Bb
BBa_K349009 Version 1 (Component)
KanR-AB-BbsI (for construction of AB BioBytes 2.0)

KanR-BA-Bf
BBa_K349012 Version 1 (Component)
KanR-BA-BfuAI (for construction of BA BioBytes 2.0)

BBa_K235026
BBa_K235026 Version 1 (Component)
[K235022][K235001] (NAND gate control test, pLac positive control)

BBa_K235027
BBa_K235027 Version 1 (Component)
[K235024][K235000] (NAND gate control test, arabinose positive control)

BBa_K1688005
BBa_K1688005 Version 1 (Component)
dTomato and ModLac with HlyA tag (inc RBS, NahR/Psal promoter system)

BBa_K1688009
BBa_K1688009 Version 1 (Component)
dTomato, ModLac and Dioxygenase (inc RBS, NahR/Psal promoter system)

BBa_K395163
BBa_K395163 Version 1 (Component)
KanR activated by RhlR and C4HSL(AHL of Rhl family)

BBa_K581017
BBa_K581017 Version 1 (Component)
araC-pBAD-supD-nahR-psal-supD-pT7-rbs-gfp

BBa_K1716002
BBa_K1716002 Version 1 (Component)
NahR Biosensor for detection of acetylsalicylic acid/aspirin with blue chromoprotein reporter

BBa_K737048
BBa_K737048 Version 1 (Component)
The leading sequence involved in the nanC transcript which can act as the target of the sRNA, spot42

BBa_K235025
BBa_K235025 Version 1 (Component)
[K235022][K235000] (NAND gate, pAra and pLac input signal control, mCherry output signal)

BBa_K1795300
BBa_K1795300 Version 1 (Component)
KanR coding region

BBa_K132005
BBa_K132005 Version 1 (Component)
kanR+lacI+Plac+kanR

BBa_K132006
BBa_K132006 Version 1 (Component)
kanR+lacI+Plac+kanR

BBa_K132004
BBa_K132004 Version 1 (Component)
kanR+lacI+Plac+kanR

BBa_K132002
BBa_K132002 Version 1 (Component)
kanR+lacI+Plac

BBa_K132016
BBa_K132016 Version 1 (Component)
luxI+KanR-LVA+LacI+PL+KanR-LVA+aiiA+terminator

KanR-AB-Bs
BBa_K349007 Version 1 (Component)
KanR-AB-BsaI (for construction of AB BioBytes 2.0)

KanR-BA-Bs
BBa_K349008 Version 1 (Component)
KanR-BA-BsaI (for construction of BA BioBytes 2.0)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.