BBa_K1321102BBa_K1321102 Version 1 (Component)Phytochelatin (PC) EC20 + CBDcex with T7 promoter
RBS-pcyABBa_K193402 Version 1 (Component)RBS is located upstream of pcyA
BBa_K176248BBa_K176248 Version 1 (Component)Low input score automatic selector (with reporter): PoPS->mRFP->CI Inverter (cI-LVA+pCI)->K176229
BBa_K1157006BBa_K1157006 Version 1 (Component)pRhl-RBS-mCherry-tt-Pc-RBS-RhlR-tt
BBa_K2176001BBa_K2176001 Version 1 (Component)Cassette for the constitutive production of GAL4-KaiCp and LexA-SasA, linked by a self-cleaving P2A
BBa_K572011BBa_K572011 Version 1 (Component)Mouse B-carotene dioxygenase generator (w/o Double Terminator)
BBa_K1321113BBa_K1321113 Version 1 (Component)Phytochelatin (PC) EC20 + linker-CBDcipA-linker with LacI promoter
BBa_K1321106BBa_K1321106 Version 1 (Component)Linker-CBDcipA-linker + Phytochelatin (PC) EC20 with T7 promoter
BBa_K1321103BBa_K1321103 Version 1 (Component)Phytochelatin (PC) EC20 + linker-CBDcipA-linker with T7 promoter
BBa_K176249BBa_K176249 Version 1 (Component)Low input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240
BBa_K1075010BBa_K1075010 Version 1 (Component)pC-RBS32-FKBP12-EcsspB[XB]-Term-pB-RBS32-EcsspB[Core]-FRB-Term-Term (Rapamycin inducable EcsspB)
BBa_K737000BBa_K737000 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_K1159310BBa_K1159310 Version 1 (Component)34 AA flexible linker with C-terminal TEV cleavage site (GGGGS)x5-TEV-site-linker) in RFC[25]
BBa_K1321117BBa_K1321117 Version 1 (Component)CBDcenA-linker fused to Phytochelatin (PC) EC20 with LacI promoter
BBa_K1321107BBa_K1321107 Version 1 (Component)CBDcen-linker fused to Phytochelatin (PC) EC20 with T7 promoter
BBa_K737001BBa_K737001 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_K1022129BBa_K1022129 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Magainin II:RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly
BBa_K1022128BBa_K1022128 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly
BBa_K1022127BBa_K1022127 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lys
BBa_I751201BBa_I751201 Version 1 (Component)pcI-luxI on pSB4
BBa_K1920000BBa_K1920000 Version 1 (Component)Pcar-RBS-RFP-TT
BBa_K1199046BBa_K1199046 Version 1 (Component)DhaA31-P2A-HheCW249P 1,2,3-tricholoropropane(TCP)->Glycerol
BBa_K1462400BBa_K1462400 Version 1 (Component)pGAL1+PRK+GBD-ligand+ADH1+pTDH3+RuBisCo+SH3-ligand+ADH1+pTDH3+CA+PDZ-ligand+ADH1
BBa_M11409BBa_M11409 Version 1 (Component)Promoter of pglnBP2 gene in Synechocystis sp. PCC 6803.
BBa_K1222004BBa_K1222004 Version 1 (Component)pCam(T7 promoter+lac operator+CamR antisense 2+T7 terminator)
BBa_M11407BBa_M11407 Version 1 (Component)Type 1 promoter of hspA gene in Synechocystis sp. PCC 6803
BBa_M11408BBa_M11408 Version 1 (Component)Type 1 promoter of sigA gene in Synechocystis sp. PCC 6803
BBa_M11404BBa_M11404 Version 1 (Component)Type 1 promoter of psbA2 gene in Synechocystis sp. PCC 6803
BBa_M11402BBa_M11402 Version 1 (Component)5' UTR and RBS of psbA2 gene in Synechocystis sp. PCC 6803
BBa_K2170132BBa_K2170132 Version 1 (Component)C-terminal intermediate of eukaryotic hypoxie sensor (VEGF-p2A-PDGF_polyA) in RFC[10]
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.