Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Danqian Liu, Hao Jiang
Date created: 2009-10-14 11:00:00
Date modified: 2015-05-08 01:11:05
Low input score automatic selector (with reporter): PoPS->mRFP->CI Inverter (cI-LVA+pCI)->K176229
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K176248_sequence (Version 1) |
Description
PoPS->mRFP->cI-LVA+pCI->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)Cell Death = (Cell Density * (pCon 0.15 + Population Average (1 / (1 + Input PoPS))) + Input AHL) * Input PoPS
Cells with relative low input score in the population will be selected, cells with relative high input score will die.
The first luxI-LVA is used to calculate average score in the population.
mRFP is input score reporter.
Notes
PoPS->mRFP->cI-LVA+pCI->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)Input1: PoPS
Input2: AHL
Output1: AHL
Output2: Cell Density
Output3: mRFP
Design:
Input1 PoPS generates Output3 mRFP and CI-LVA.
pCI generates LuxI-LVA and TetR-LVA, repressed by CI-LVA.
pCon 0.15(K176008) also generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.
pCon 0.70(J23101) generates LuxR.
pLux/Tet(K176000) generates lacZalpha-ccdB (weak RBS) to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input2 AHL), repressed by TetR-LVA and no aTc.
Source
K176224 K176226| Access | Instance | Definition |
| public public | BBa_K176224 BBa_K176226 | BBa_K176224 BBa_K176226 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K176224 BBa_K176226 | 1,2388 2397,5797 | BBa_K176224 BBa_K176226 |