BBa_J119107BBa_J119107 Version 1 (Component)pT7-RBS-GFP-pLac+tRNA CCACC+pLac+tRNA AGGAC+pLac+tRNA CUACC
BBa_K1974033BBa_K1974033 Version 1 (Component)T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag
BBa_K362007BBa_K362007 Version 1 (Component)This composite part has the promoter ,ahpC-promoter controlled by OxyR protein, and GFP - T-T
BBa_K1053209BBa_K1053209 Version 1 (Component)Pconst.- RBS-YF1/FixJ-DT- Pfixk2- taRNA- DT- Pconst.- HHR*- GFP- DT
BBa_R4030BBa_R4030 Version 1 (Component)PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)
BBa_K1753000BBa_K1753000 Version 1 (Component)the omega subunit of E.coli RNA polymerase with T7 promoter, RBS and terminator(BBa_B0014)
pSBBs4SBBa_K823022 Version 1 (Component)pSB<sub>Bs</sub>4S: Empty backbone for integration into <i/>Bacillus subtilis thrC</i> locus
pSBBs1CBBa_K823023 Version 1 (Component)pSB<sub>Bs</sub>1C: Empty backbone for integration into <i>Bacillus subtilis</i> <i>amyE</i> locus
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
BBa_J24822BBa_J24822 Version 1 (Component)Same as J24819 but with the error at the luc-terminator junction fixed
Bacillus subtilis Collectionbsu_collection Version 1 (Collection)This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.
BBa_K165100BBa_K165100 Version 1 (Component)Gli1 bs + LexA bs + mCYC + LexA repressor (mCherryx2 tagged) on pRS304*
BBa_K079021BBa_K079021 Version 1 (Component)LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La
BBa_K165101BBa_K165101 Version 1 (Component)Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS304*
BBa_K180005BBa_K180005 Version 1 (Component)GoL - Primary plasmid (part 1)/RPS - Paper primary plasmid (part 1) [LuxR generator]
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.