BBa_I715068
BBa_I715068 Version 1 (Component)
Partially flippable reverse Tet.

BBa_K415005
BBa_K415005 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term

Stalker
BBa_J06001 Version 1 (Component)
Tet repressible luxI generator ( LVA+ )

BBa_K1053303
BBa_K1053303 Version 1 (Component)
PlacO-1-RBS-tetR-DT-PcI-RBS-lacI-DT-PtetO-1-RBS-cI-DT

BBa_I720010
BBa_I720010 Version 1 (Component)
Ara landing pad (pBBLP 8)

BBa_K329003
BBa_K329003 Version 1 (Component)
Strong RBS (B0034) - Tetracycline resistance protein TetA(C) (J31007)

BBa_S03641
BBa_S03641 Version 1 (Component)
HixC : TetB-RBS_rev-HixC

BBa_J06101
BBa_J06101 Version 1 (Component)
Tet repressible lasI generator ( LVA+ )

BBa_I759033
BBa_I759033 Version 1 (Component)
cis1-repressed, tet-regulated YFP

BBa_I759045
BBa_I759045 Version 1 (Component)
cis7-repressed, tet-regulated YFP

BBa_I759043
BBa_I759043 Version 1 (Component)
cis6-repressed, tet-regulated YFP

BBa_I759037
BBa_I759037 Version 1 (Component)
cis3-repressed, tet-regulated YFP

BBa_I759047
BBa_I759047 Version 1 (Component)
cis8-repressed, tet-regulated YFP

BBa_I759041
BBa_I759041 Version 1 (Component)
cis5-repressed, tet-regulated YFP

BBa_I759035
BBa_I759035 Version 1 (Component)
cis2-repressed, tet-regulated YFP

BBa_K1444011
BBa_K1444011 Version 1 (Component)
Composite promoter and consensus B. subtilis RBS - pTetR

BBa_S03736
BBa_S03736 Version 1 (Component)
pLac-lox-RBS-Tet (in pSB1A2)

BBa_K208013
BBa_K208013 Version 1 (Component)
Tet Repressible Promoter (BBa_R0040) and RBS (BBa_B0034)

BBa_K1444014
BBa_K1444014 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - pTetR x2

BBa_S03766
BBa_S03766 Version 1 (Component)
RBS-Kan-RBS-Tet-RBS-RFP (pSB1A7)

BBa_K196013
BBa_K196013 Version 1 (Component)
Hybrid promoter (Lux cassette + c2 P22 promoter) + RBS + LuxR + ter

BBa_S03737
BBa_S03737 Version 1 (Component)
pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)

BBa_K415011
BBa_K415011 Version 1 (Component)
PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.