BBa_K1333005
BBa_K1333005 Version 1 (Component)
M13 bacteriophage g8p with a FLAG tag on C-terminal

BBa_I764000
BBa_I764000 Version 1 (Component)
production of tetR under the arabinose regulated promoter (AraC)

SAR
BBa_K2015000 Version 1 (Component)
Self-assembling region (SAR) for the circularization of proteins

BBa_K078006
BBa_K078006 Version 1 (Component)
One part of the initial dioxygenase in dioxin degradation pathway

BBa_K779315
BBa_K779315 Version 1 (Component)
5' Hammerhead with loss-of-function mutation MammoBlock

BBa_K779316
BBa_K779316 Version 1 (Component)
3' Hammerhead with loss-of-function mutation MammoBlock

BBa_K617003
BBa_K617003 Version 1 (Component)
R6K origin of replication

BBa_K1509000
BBa_K1509000 Version 1 (Component)
Coding for the trans-acting regulator SmtB

BBa_K1412600
BBa_K1412600 Version 1 (Component)
Endow the CL-1(E.coli engineered strain) the ability of chemotaxis and quorum sensing

BBa_K1994027
BBa_K1994027 Version 1 (Component)
Com-Sigma 54 fusion protein

BBa_K1994023
BBa_K1994023 Version 1 (Component)
PP7-Sigma 54 fusion protein

BBa_K1994030
BBa_K1994030 Version 1 (Component)
MS2-Sigma 54 fusion protein

BBa_K1399007
BBa_K1399007 Version 1 (Component)
GFP (mut3b) with SsrA-DAS+2 degradation tag

BBa_K1689006
BBa_K1689006 Version 1 (Component)
Coding sequence of FKBP-Cluc394

BBa_K1361007
BBa_K1361007 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative

BBa_K1189009
BBa_K1189009 Version 1 (Component)
Beta-lactamase with His Tag

epsE 5 IS
BBa_K143005 Version 1 (Component)
5??? Integration Sequence for the epsE locus of B. subtilis

epsE 3 IS
BBa_K143006 Version 1 (Component)
3??? Integration Sequence for the epsE locus of B. subtilis

pSB1AK3X
BBa_J18905 Version 1 (Component)
pSB1AK3X Freiburg Fusion -> BioFusion conversion plasmid

pSB1AC3X
BBa_J18904 Version 1 (Component)
pSB1AC3X Freiburg Fusion -> BioFusion conversion plasmid

pSB1AT3X
BBa_J18906 Version 1 (Component)
pSB1AT3X Freiburg Fusion -> BioFusion conversion plasmid

BBa_K115035
BBa_K115035 Version 1 (Component)
Expression of luciferase with RNA-thermometer K115017

BBa_K115031
BBa_K115031 Version 1 (Component)
Expression of luciferase with RNA-thermometer K115003

BBa_K115034
BBa_K115034 Version 1 (Component)
Expression of luciferase with RNA-thermometer K115016

BBa_K115033
BBa_K115033 Version 1 (Component)
Expression of luciferase with RNA-thermometer K115009

BBa_K648131
BBa_K648131 Version 1 (Component)
Pr of Or tester with cI repressor

BBa_K115032
BBa_K115032 Version 1 (Component)
Expression of luciferase with RNA-thermometer K115008

BBa_K115030
BBa_K115030 Version 1 (Component)
Expression of luciferase with RNA-thermometer K115002

BBa_K115029
BBa_K115029 Version 1 (Component)
Expression of luciferase with RNA-thermometer K115001

BBa_K176100
BBa_K176100 Version 1 (Component)
constitutive promoter family member J23100 (pCon 1.00) with primer VR3 site

BBa_K176099
BBa_K176099 Version 1 (Component)
constitutive promoter family member J23119 (pCon max) with primer VR10 site

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1719009
BBa_K1719009 Version 1 (Component)
Circuit encoding leucine dehydrogenase and formate dehydrogenase

BBa_K1719010
BBa_K1719010 Version 1 (Component)
Circuit encoding leucine dehydrogenase and formate dehydrogenase

BBa_K582000
BBa_K582000 Version 1 (Component)
500bp of Spacer/Repeat Region of the CRISPR genome present in Strep. thermophilus LMD9 strain

BBa_K880003
BBa_K880003 Version 1 (Component)
Asymmetrically digestible reporter, same orientation of inverted repeats as K137058 (external sites

BBa_K2047006
BBa_K2047006 Version 1 (Component)
Stem-loop with free energy of -38.5kcal/mol measured by Mfold

BBa_K2082111
BBa_K2082111 Version 1 (Component)
Assembly of six mutator genes, each with weak promoter; terminal terminator

BBa_K2047005
BBa_K2047005 Version 1 (Component)
Stem-loop with free energy of -30.1kcal/mol measured by Mfold

BBa_K2047004
BBa_K2047004 Version 1 (Component)
Stem-loop with free energy of -38.7kcal/mol measured by Mfold

BBa_K2047007
BBa_K2047007 Version 1 (Component)
Stem-loop with free energy of -34.4kcal/mol measured by Mfold

BBa_K2047008
BBa_K2047008 Version 1 (Component)
Stem-loop with free energy of -38.8kcal/mol measured by Mfold

BBa_K648130
BBa_K648130 Version 1 (Component)
Pr of Or tester with cI repressor with very weak RBS

BBa_K648129
BBa_K648129 Version 1 (Component)
Pr of Or tester with cI repressor with slightly weak RBS

BBa_K833013
BBa_K833013 Version 1 (Component)
Lactate sensor derived from the AraF protein of E. coli

CI-tag
BBa_K1145007 Version 1 (Component)
A protein which can inhibit the expression of Pλ.

Plac/ara-1
BBa_K1145006 Version 1 (Component)
Drive the expression of LuxRI in pLuxRI2 and its derivatives.

Plsr
BBa_K1145004 Version 1 (Component)
Take effect when the density of bacteria hits certain value.

BBa_K1797014
BBa_K1797014 Version 1 (Component)
Gam and Bet-Two Components of the Red Recombination System

FixJ
BBa_K592005 Version 1 (Component)
FixJ, the response regulator to YF1