BBa_K078006BBa_K078006 Version 1 (Component)One part of the initial dioxygenase in dioxin degradation pathway
BBa_K779315BBa_K779315 Version 1 (Component)5' Hammerhead with loss-of-function mutation MammoBlock
BBa_K779316BBa_K779316 Version 1 (Component)3' Hammerhead with loss-of-function mutation MammoBlock
BBa_K617003BBa_K617003 Version 1 (Component)R6K origin of replication
BBa_K1509000BBa_K1509000 Version 1 (Component)Coding for the trans-acting regulator SmtB
BBa_K1412600BBa_K1412600 Version 1 (Component)Endow the CL-1(E.coli engineered strain) the ability of chemotaxis and quorum sensing
BBa_K1994027BBa_K1994027 Version 1 (Component)Com-Sigma 54 fusion protein
BBa_K1994023BBa_K1994023 Version 1 (Component)PP7-Sigma 54 fusion protein
BBa_K1994030BBa_K1994030 Version 1 (Component)MS2-Sigma 54 fusion protein
BBa_K1399007BBa_K1399007 Version 1 (Component)GFP (mut3b) with SsrA-DAS+2 degradation tag
BBa_K1689006BBa_K1689006 Version 1 (Component)Coding sequence of FKBP-Cluc394
BBa_K1361007BBa_K1361007 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative
BBa_K1189009BBa_K1189009 Version 1 (Component)Beta-lactamase with His Tag
epsE 5 ISBBa_K143005 Version 1 (Component)5??? Integration Sequence for the epsE locus of B. subtilis
epsE 3 ISBBa_K143006 Version 1 (Component)3??? Integration Sequence for the epsE locus of B. subtilis
pSB1AK3XBBa_J18905 Version 1 (Component)pSB1AK3X Freiburg Fusion -> BioFusion conversion plasmid
pSB1AC3XBBa_J18904 Version 1 (Component)pSB1AC3X Freiburg Fusion -> BioFusion conversion plasmid
pSB1AT3XBBa_J18906 Version 1 (Component)pSB1AT3X Freiburg Fusion -> BioFusion conversion plasmid
BBa_K115035BBa_K115035 Version 1 (Component)Expression of luciferase with RNA-thermometer K115017
BBa_K115031BBa_K115031 Version 1 (Component)Expression of luciferase with RNA-thermometer K115003
BBa_K115034BBa_K115034 Version 1 (Component)Expression of luciferase with RNA-thermometer K115016
BBa_K115033BBa_K115033 Version 1 (Component)Expression of luciferase with RNA-thermometer K115009
BBa_K648131BBa_K648131 Version 1 (Component)Pr of Or tester with cI repressor
BBa_K115032BBa_K115032 Version 1 (Component)Expression of luciferase with RNA-thermometer K115008
BBa_K115030BBa_K115030 Version 1 (Component)Expression of luciferase with RNA-thermometer K115002
BBa_K115029BBa_K115029 Version 1 (Component)Expression of luciferase with RNA-thermometer K115001
BBa_K176100BBa_K176100 Version 1 (Component)constitutive promoter family member J23100 (pCon 1.00) with primer VR3 site
BBa_K176099BBa_K176099 Version 1 (Component)constitutive promoter family member J23119 (pCon max) with primer VR10 site
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K1719009BBa_K1719009 Version 1 (Component)Circuit encoding leucine dehydrogenase and formate dehydrogenase
BBa_K1719010BBa_K1719010 Version 1 (Component)Circuit encoding leucine dehydrogenase and formate dehydrogenase
BBa_K582000BBa_K582000 Version 1 (Component)500bp of Spacer/Repeat Region of the CRISPR genome present in Strep. thermophilus LMD9 strain
BBa_K880003BBa_K880003 Version 1 (Component)Asymmetrically digestible reporter, same orientation of inverted repeats as K137058 (external sites
BBa_K2047006BBa_K2047006 Version 1 (Component)Stem-loop with free energy of -38.5kcal/mol measured by Mfold
BBa_K2082111BBa_K2082111 Version 1 (Component)Assembly of six mutator genes, each with weak promoter; terminal terminator
BBa_K2047005BBa_K2047005 Version 1 (Component)Stem-loop with free energy of -30.1kcal/mol measured by Mfold
BBa_K2047004BBa_K2047004 Version 1 (Component)Stem-loop with free energy of -38.7kcal/mol measured by Mfold
BBa_K2047007BBa_K2047007 Version 1 (Component)Stem-loop with free energy of -34.4kcal/mol measured by Mfold
BBa_K2047008BBa_K2047008 Version 1 (Component)Stem-loop with free energy of -38.8kcal/mol measured by Mfold
BBa_K648130BBa_K648130 Version 1 (Component)Pr of Or tester with cI repressor with very weak RBS
BBa_K648129BBa_K648129 Version 1 (Component)Pr of Or tester with cI repressor with slightly weak RBS
BBa_K833013BBa_K833013 Version 1 (Component)Lactate sensor derived from the AraF protein of E. coli
CI-tagBBa_K1145007 Version 1 (Component)A protein which can inhibit the expression of Pλ.
Plac/ara-1BBa_K1145006 Version 1 (Component)Drive the expression of LuxRI in pLuxRI2 and its derivatives.
PlsrBBa_K1145004 Version 1 (Component)Take effect when the density of bacteria hits certain value.
BBa_K1797014BBa_K1797014 Version 1 (Component)Gam and Bet-Two Components of the Red Recombination System
FixJBBa_K592005 Version 1 (Component)FixJ, the response regulator to YF1
sbks activitiessbksactivities_collection Version 1 (Collection)Activities linked to the SBKS project
BBa_K1689005BBa_K1689005 Version 1 (Component)Coding sequence of FKBP-Cluc398
BBa_K1689002BBa_K1689002 Version 1 (Component)Coding sequence of Cluc-STV