BBa_J70605
BBa_J70605 Version 1 (Component)
J70590 cut with BsaXI and with GSGSGS inserts (J70556-fi,ri) added

BBa_K318518
BBa_K318518 Version 1 (Component)
Bile Salt Induced Lysis Cassette

BBa_K1189000
BBa_K1189000 Version 1 (Component)
TALE-A with a his tag under a lacI promoter

BBa_K952007
BBa_K952007 Version 1 (Component)
IPTG induc. promoter-B0034-YF1-B0034-FixJ-PfixK2-Lysis Cassette

BBa_K638201
BBa_K638201 Version 1 (Component)
Arabinose inducible Poly-His Reflectin A1 generator

BBa_J70606
BBa_J70606 Version 1 (Component)
J70590 cut with BsaXI and with dead fusion inserts (J70556d-fi,ri) added

BBa_K1189001
BBa_K1189001 Version 1 (Component)
TALE-B with a his 6 tag under a lacI promoter

BBa_K364331
BBa_K364331 Version 1 (Component)
TRE-PolyA in pBSB1A3

BBa_K1088010
BBa_K1088010 Version 1 (Component)
E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib

BBa_K1075003
BBa_K1075003 Version 1 (Component)
Promoter(const.)-AraC-Term-pBAD-RBS34 (Arabinose inducable promoter system)

BBa_K1413041
BBa_K1413041 Version 1 (Component)
Mutation of OriVR6Kgamma origin of replication (ori)

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

BBa_K1189031
BBa_K1189031 Version 1 (Component)
TALE-A linked to beta-lactamase with a his tag under a lacI promoter

TALEA-dife
BBa_K1189021 Version 1 (Component)
TALE-A ferritin di-subunit direct fusion w/ hisTag under lacI promoter, and linker

BBa_K332034
BBa_K332034 Version 1 (Component)
Constitutive promoter + 37℃ induced RBS + tetR + double terminator

BBa_K1154006
BBa_K1154006 Version 1 (Component)
Mating pheromone-induced IGPD and constitutive LDH expression in yeast

BBa_K1861020
BBa_K1861020 Version 1 (Component)
Fusion protein of the TALE constant domain and a SpyTag in an expression cassette

BBa_K1189030
BBa_K1189030 Version 1 (Component)
TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_K1053006
BBa_K1053006 Version 1 (Component)
PBAD-TR(42)-HHR-taR12-DT

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K1053005
BBa_K1053005 Version 1 (Component)
PBAD- TR-HHR*(RBS)-GFPuv-DT

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.