BBa_K332034
BBa_K332034 Version 1 Component
This part has been discontinued.
Please consider using BBa_K332031 as a replacement.
Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: KUO, CHIA-CHUN
Date created: 2010-10-23 11:00:00
Date modified: 2015-05-08 01:12:05
Constitutive promoter + 37℃
induced RBS + tetR + double terminator
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K332034_sequence (Version 1) |
Description
To regulate following genes by temperature.To maintain cells at 37?? C ↑ : the tetR protein be generated and repress Ptet's circuit.
To release cells at 37?? C ↓ : the tetR protein not be generated and do not repress Ptet's circuit.
The theoretical switching temperature of BBa_k115002, which was designed by Team TUDelft in 2008, is 37℃.
Thus, our thermosensing device A can be induced at this temperature. (The switching temperature can be modified by changing RBS according to different conditions.)
Once being swiched on, tetR will inhibit the corresponding promoter, pTet, which enables us to regulate the expression of cry gene and quorum sensing gene devices.
Notes
To regulate following genes by temperature.Source
the 37℃ induced RBS is from iGEM08_TUDelft| Access | Instance | Definition |
| public public public public | BBa_J23101 BBa_K115002 BBa_C0040 BBa_J61048 | BBa_J23101 BBa_K115002 tetR BBa_J61048 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23101 BBa_K115002 BBa_C0040 BBa_J61048 | 1,35 44,95 102,761 795,907 | BBa_J23101 BBa_K115002 tetR BBa_J61048 |