BBa_K737001BBa_K737001 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_S03674BBa_S03674 Version 1 (Component)(-1,2) HixC-pBad<sub>rev</sub>-HixC : RBS-TetF-HixC
BBa_K1676120BBa_K1676120 Version 1 (Component)Mutant 16 of Lactate Dehydrogenase
BBa_K1676114BBa_K1676114 Version 1 (Component)Mutant 10 of Lactate Dehydrogenase
BBa_K1361007BBa_K1361007 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative
BBa_K2082252BBa_K2082252 Version 1 (Component)RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI
BBa_K1228001BBa_K1228001 Version 1 (Component)A fragment of loctoferrin
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K2041010BBa_K2041010 Version 1 (Component)plac-RBS-Bxb1-T-plux-RBS-luxR-T-plux-recognition site of Bxb1-RBS-luxI-RBS-GFP-T
BBa_K809108BBa_K809108 Version 1 (Component)Efficiency test of Q0255 Terminator
RFP_SPtim2BBa_K809303 Version 1 (Component)RFP + signal peptide of TIM21
BBa_K337088BBa_K337088 Version 1 (Component)Fragment 7 of wt AAV6
BBa_K337060BBa_K337060 Version 1 (Component)Fragment 3 of wt AAV1
BBa_K1413042BBa_K1413042 Version 1 (Component)OriVR6Kgamma origin of replication (ori) 2
BBa_J22121BBa_J22121 Version 1 (Component)Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3
BBa_K1523102BBa_K1523102 Version 1 (Component)The translational unit of yieF
T25 domainBBa_K1088056 Version 1 (Component)T25 domain of CyaA from Bordetella pertussis
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_M31786BBa_M31786 Version 1 (Component)1st half of Gene III- preBamHI cut
BBa_K648103BBa_K648103 Version 1 (Component)RecA with mutation of Arg 243
CsgDBBa_K1019001 Version 1 (Component)CsgD: positive regulator of curlin genes
BBa_K648102BBa_K648102 Version 1 (Component)RecA with mutation of Lys 286
BBa_K1114003BBa_K1114003 Version 1 (Component)The MoClo format of BBa_J23103 with AB fusion sites.
PrtDEFBBa_K258007 Version 1 (Component)Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
BBa_K831011BBa_K831011 Version 1 (Component)istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12
BBa_K831012BBa_K831012 Version 1 (Component)istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12
BBa_I733005BBa_I733005 Version 1 (Component)Produce GFP in presence of AHL
BBa_K1725317BBa_K1725317 Version 1 (Component)Member of the RBS library - derived from BBa_B0032
BBa_I12026BBa_I12026 Version 1 (Component)Test of BBa_R0011 (LacI regulated) using YFP