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BBa_K2041010

BBa_K2041010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2041010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: ZHOU CHUANGUI

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 11:41:08

plac-RBS-Bxb1-T-plux-RBS-luxR-T-plux-recognition site of Bxb1-RBS-luxI-RBS-GFP-T

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K2041010_sequence (Version 1)

Description

The Bxb1 recombinase can bind to the binding site of Bxb1 and make GFP express,but the level of expression is not high.Use an amplifying system (BBa_K546003) can increase the level of expression of GFP.

Notes

This is a composite part including Bxb1 rocombinase coding region encoded in part BBa_K907000,it also includes the recognition site of Bxb1 rocombinase and an amplifying system.

Source

All of these parts are from Kit Plate of iGEM foundation.

Components

Access	Instance	Definition
public public public public public public public public public public public public	BBa_R0010 BBa_B0034 BBa_K907000 BBa_B0015 BBa_B0015 BBa_K546003 BBa_I11032 BBa_J23104 BBa_I11033 BBa_B0034 BBa_C0061 BBa_E0840	LacI BBa_B0034 Bxb1_Int BBa_B0015 BBa_B0015 BBa_K546003 P22 attB BBa_J23104 P22 attP BBa_B0034 luxI GFP genera

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010 BBa_B0034 BBa_K907000 BBa_B0015 BBa_B0015 BBa_K546003 BBa_I11032 BBa_J23104 BBa_I11033 BBa_B0034 BBa_C0061 BBa_E0840	1,200 209,220 227,1729 1738,1866 2841,2969 1875,3032 3041,3067 3076,3110 3119,3378 3387,3398 3405,4022 4056,4933	LacI BBa_B0034 Bxb1_Int BBa_B0015 BBa_B0015 BBa_K546003 P22 attB BBa_J23104 P22 attP BBa_B0034 luxI GFP genera

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K2041010/1