BBa_K1444010
BBa_K1444010 Version 1 (Component)
Composite promoter and weak B. subtilis RBS - C1-434

BBa_K883704
BBa_K883704 Version 1 (Component)
IPTG induced promoter + GFP fused to a coiled coil

recNp
BBa_K629002 Version 1 (Component)
recNp, could be started with exposure to irradiation, UV, nalidixic acid

BBa_K1334020
BBa_K1334020 Version 1 (Component)
a signal complex to test whether the lux system works

BBa_K976009
BBa_K976009 Version 1 (Component)
TDH3 Promoter + Yeast Kozak + FGFR-1/FRS2 Complex +TEF1 Terminator

BBa_K2088012
BBa_K2088012 Version 1 (Component)
MhbRpt is a complex promoter which can be induced by 3-HBA.

cN
BBa_K2027003 Version 1 (Component)
Bacterial Collagen with Elastin Cross-linker and Homotrimeric Coiled-Coil Domain

c2
BBa_K2027005 Version 1 (Component)
Bacterial Collagen with Elastin Cross-linker and Coiled-Coil Heterotrimerization Domain 2

c3
BBa_K2027006 Version 1 (Component)
Bacterial Collagen with Elastin Cross-linker and Coiled-Coil Heterotrimerization Domain 3

BBa_K1935013
BBa_K1935013 Version 1 (Component)
White Collar Complex under control of C.elegans's promoter (pUNC119::WC1::P2A::WC2)

BBa_K2027046
BBa_K2027046 Version 1 (Component)
Bacterial Collagen with Homotrimeric Coiled-Coil, Elastin Cross-linker, and mRNA Circularization

BBa_K896907
BBa_K896907 Version 1 (Component)
An and gate promoter which could promote colicin

BBa_K883703
BBa_K883703 Version 1 (Component)
IPTG induced promoter + GFP with a His-tag fused to a coiled coil

BBa_K883705
BBa_K883705 Version 1 (Component)
IPTG induced promoter + GFP with a His-tag fused to a coiled coil

BBa_K1942000
BBa_K1942000 Version 1 (Component)
A shRNA corresponding DNA sequence for KRAS which could silence the gene

BBa_K987001
BBa_K987001 Version 1 (Component)
This is a composite part which has the function to invert the temperature activation by the part: BB

BBa_K737071
BBa_K737071 Version 1 (Component)
phoR, a histine kinase coding gene involved in phoBR regulon, is the regulatory protein that could a

BBa_K1790000
BBa_K1790000 Version 1 (Component)
biosensors detect food allergens. A proteins conformation change in response to ligand binding coupl

BBa_K737070
BBa_K737070 Version 1 (Component)
phoR, a histine kinase coding gene involved in phoBR regulon, is the regulatory protein that could a

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.