recNp

BBa_K629002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K629002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zilong WANG, Yi ZHENG

Date created: 2011-10-02 11:00:00

Date modified: 2015-05-08 01:12:56

recNp, could be started with exposure to irradiation, UV, nalidixic acid

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K629002_sequence (Version 1)

Description

Name: recNp
+1: 2749781
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 36
Sequence: aattattctaattttacgccagcctctttactgtatataaaaccagtttatactgtacacAataacagtaatggtttttca
-35 -10 +1
Note(s): We assigned a putative transcription start site to this promoter based on the observation that the majority of the promoters, whose transcription start sites were determined experimentally, present a distance of 6 nucleotides between the transcription start site and the -10 box.
Evidence(s): [HIPP] Human inference of promoter position
Reference(s): [1] Rostas K., et al., 1987

Notes

the -35 and -10 district, the RBS

Source

E. Coli BL21 plys genome

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
fwd primer	1,18	primer_binding_site, feature/primer_binding
-35 box	58,63	conserved_region, feature/conserved
-10 box	77,85	conserved_region, feature/conserved
recNp	108,125	primer_binding_site, feature/primer_binding
RBS	116,119	ribosome_entry_site, feature/rbs
LexA	82,101	feature/binding, non_covalent_binding_site

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K629002/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //classic/regulatory/other
• iGEM Parts Registry