BBa_K1366101
BBa_K1366101 Version 1 (Component)
Genetic construct for lpp gene deletion (C1)

BBa_K598015
BBa_K598015 Version 1 (Component)
T7-promoter+TPP Up-regulated Hammerhead Ribozyme 1.20 with Native RBS+GFP+T7-terminator

BBa_K598016
BBa_K598016 Version 1 (Component)
T7-promoter+TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+GFP+T7-terminator

BBa_K195202
BBa_K195202 Version 1 (Component)
Lpp-OmpA-linker+alpha-V integrin gene+terminator

BBa_K195203
BBa_K195203 Version 1 (Component)
RBS+Lpp-OmpA-linker+alpha-V integrin gene+terminator

BBa_K1231003
BBa_K1231003 Version 1 (Component)
Asr-64-Lpp-RBS-GFP is a dual-state construct

BBa_K187006
BBa_K187006 Version 1 (Component)
Sigma 70 50% promoter in pAB, Biobytes plasmid

BBa_K1676088
BBa_K1676088 Version 1 (Component)
Mutant 12 of LDH and wildtype LDP

BBa_K1676091
BBa_K1676091 Version 1 (Component)
Mutant 15 of LDH and wildtype LDP

BBa_K1676086
BBa_K1676086 Version 1 (Component)
Mutant 10 of LDH and wildtype LDP

BBa_K1676080
BBa_K1676080 Version 1 (Component)
Mutant 4 of LDH and wildtype LDP

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.