Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ooi Kean Hean
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-25 02:12:47
Mutant 4 of LDH and wildtype LDP
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1676080_sequence (Version 1) |
Description
This construct is an operon for expression of our mutant LDH and wildtype LDP.Notes
Primers were designed to have the start and stop codon so that they will not be mutated during PCRSource
Wild type LDH and LDP was obtained from a Pseudomonas Strain and random mutagenesis was carried out by PCR to make lots of Mutant LDH. PCR fragments carrying the mutants were then ligated to the wild type LDP to make a complete operon.| Access | Instance | Definition |
| public public public public public public | BBa_R0011 BBa_B0034 BBa_K1676107 BBa_B0034 BBa_K1676076 BBa_B0015 | lacI+pL BBa_B0034 BBa_K1676107 BBa_B0034 BBa_K1676076 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 BBa_B0034 BBa_K1676107 BBa_B0034 BBa_K1676076 BBa_B0015 | 1,54 64,75 82,1227 1236,1247 1254,2942 2951,3079 | lacI+pL BBa_B0034 BBa_K1676107 BBa_B0034 BBa_K1676076 BBa_B0015 |