Types | DnaRegion
|
Roles | CDS
Coding
|
Sequences | BBa_K1676107_sequence (Version 1)
|
Description
The is a mutant of lactate dehydrogenase which contain mutation to these positions: S6Y, R10P, Q14K, L60S, A95D, F126L, K132I, R138P, A177T, W193R, W233S, V271F, T311I
Notes
Primers were designed to contain the start and the stop codons so that no mutations would occur on them.
Source
Random mutagenesis via error prone PCR was carried out on the gene.