BBa_J06623
BBa_J06623 Version 1 (Component)
Construction intermediate: 434 cI QPI with Venus YFP (Q04520.J06700)

BBa_J06622
BBa_J06622 Version 1 (Component)
Construction intermediate: Lambda cI QPI with Venus YFP (Q04510.J06700)

BBa_I20300
BBa_I20300 Version 1 (Component)
RBS from T7 gene 4, optimal aligned spacing + Z0301 venus YFP

BBa_J10067
BBa_J10067 Version 1 (Component)
sacB minus RBS (based on K322921)

BBa_K2097000
BBa_K2097000 Version 1 (Component)
CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.

Venus YFP
BBa_J63001 Version 1 (Component)
enhanced version of EYFP, yeast-optimized YFP

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.