BBa_K112207
BBa_K112207 Version 1 (Component)
{int!} The integrase gene with start and stop codon, BBb format

BBa_J120010
BBa_J120010 Version 1 (Component)
Met inducible promoter linked to Pax3 coding sequence with terminator

BBa_K1499205
BBa_K1499205 Version 1 (Component)
MntH with amber stop codon with promoter for expression, no tRNA

BBa_K844005
BBa_K844005 Version 1 (Component)
Spider Silk 1x 1E Subunit "U" with Met (ATG) start codon

BBa_I756028
BBa_I756028 Version 1 (Component)
cmv - IgM signal- VH-VL-EporD2-EporTm mut- gp130i- dnaEC-VP16-SV40PA

BBa_K809603
BBa_K809603 Version 1 (Component)
Bovine Pancreatic DNase I (in yeast mt codon table)

BBa_K2066112
BBa_K2066112 Version 1 (Component)
Synthetic Enhancer Project: Ntr promoter driven NRII2302 (mut) on UNS Standard

BBa_I9001
BBa_I9001 Version 1 (Component)
QPI (mnt) B0034.C0073.B0015.R0073

BBa_J06911
BBa_J06911 Version 1 (Component)
LacI ts (mut 241) QPI regulated by lambda cI with EYFP

BBa_J06912
BBa_J06912 Version 1 (Component)
LacI ts (mut 265) QPI regulated by lambda cI with EYFP

BBa_J06902
BBa_J06902 Version 1 (Component)
LacI ts (mut 265) QPI regulated by lambda cI with mCherry

BBa_J06901
BBa_J06901 Version 1 (Component)
LacI ts (mut 241) QPI regulated by lambda cI with mCherry

BBa_J06961
BBa_J06961 Version 1 (Component)
Lambda cI/LacI ts (mut 241) RS flip-flop with EYFP

BBa_J06962
BBa_J06962 Version 1 (Component)
Lambda cI/LacI ts (mut 265) RS flip-flop with EYFP

BBa_K222003
BBa_K222003 Version 1 (Component)
Mid1 gene disrupted from 489 to 2021 nt

BBa_K222004
BBa_K222004 Version 1 (Component)
Cch1 gene disrupted from 532 to 6708 nt

BBa_K809714
BBa_K809714 Version 1 (Component)
T7 promoter + yeast mt RBS(AI1) + mtGFP

BBa_J06612
BBa_J06612 Version 1 (Component)
Construction intermediate: LacI ts (mut 265) QPI with Lambda cI regulated promoter (R0051.J06801)

BBa_J06611
BBa_J06611 Version 1 (Component)
Construction intermediate: LacI ts (mut 241) QPI with Lambda cI regulated promoter (R0051.J06800)

BBa_K112211
BBa_K112211 Version 1 (Component)
{a~int!} The integrase gene with rbs ready to be attached with stop codon, BBb format

BBa_K222002
BBa_K222002 Version 1 (Component)
project of a Mid1 gene disrupted from 489 to 2021 nt

BBa_K987001
BBa_K987001 Version 1 (Component)
This is a composite part which has the function to invert the temperature activation by the part: BB

BBa_K650000
BBa_K650000 Version 1 (Component)
D. rad MntH (DR_1709)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.