BBa_K1172305
BBa_K1172305 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of a medium Anderson promoter

BBa_K329017
BBa_K329017 Version 1 (Component)
Population counter Tetracycline resistant - PmS-MTet-LuxI

BBa_K329068
BBa_K329068 Version 1 (Component)
Population counter Tetracycline resistant - PsS-WTet-LuxI

BBa_K329019
BBa_K329019 Version 1 (Component)
Population counter Tetracycline resistant - Final PmS-MTet

BBa_K1461229
BBa_K1461229 Version 1 (Component)
resettable single counter system using ecf11

BBa_K1461240
BBa_K1461240 Version 1 (Component)
σ-Re Counter (double, using ecf11)

BBa_K1461227
BBa_K1461227 Version 1 (Component)
resettable single counter system using ecf20

BBa_K1461239
BBa_K1461239 Version 1 (Component)
σ-Re Counter (double, using ecf20)

BBa_K1172304
BBa_K1172304 Version 1 (Component)
Riboflavin synthesis gene cluster from s. oneidensis under control of T7 promoter and strong RBS

SapI (reve
BBa_B0103 Version 1 (Component)
SapI restriction enzyme site, reversed (offset cutter)

BBa_K329069
BBa_K329069 Version 1 (Component)
Population counter Tetracycline resistant after recombination - PmS-MTet-LuxI

BBa_K2088006
BBa_K2088006 Version 1 (Component)
It encodes a kind of protein named 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain. I

BBa_K1461236
BBa_K1461236 Version 1 (Component)
resettable counter device using ecf11 (w/o crRBS, taRNA)

BBa_K1461235
BBa_K1461235 Version 1 (Component)
resettable counter device using ecf20 (w/o crRBS, taRNA)

BBa_K1945002
BBa_K1945002 Version 1 (Component)
Homologous Recombination Intermediate for Counter-selection in Synechocystis sp. PCC 6803

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.