BBa_K1866010BBa_K1866010 Version 1 (Component)Promoter+RibosomeBindingSite+NosZ+SuperYellowFluorescenceProtein (in pSB1C3)
BBa_K812233BBa_K812233 Version 1 (Component)sfGFP in pCS2+ with elastase promoter
BBa_K1866002BBa_K1866002 Version 1 (Component)Promoter+RibosomeBindingSite+NrfA+SYFP (in pSB1K3)
BBa_K1484005BBa_K1484005 Version 1 (Component)AmilCP chromoprotein in BBa and MoClo standard
BBa_J72078BBa_J72078 Version 1 (Component){double terminator}, b0015 in BglBrick format
BBa_I733005BBa_I733005 Version 1 (Component)Produce GFP in presence of AHL
BBa_S03736BBa_S03736 Version 1 (Component)pLac-lox-RBS-Tet (in pSB1A2)
BBa_K1510105BBa_K1510105 Version 1 (Component)sRNA targets histidine kinase 11 mRNA in S.mutans
BBa_I733004BBa_I733004 Version 1 (Component)Produce LacZ alpha in response to AHL
BBa_K812133BBa_K812133 Version 1 (Component)sfGFP with kozak sequence for expression in Xenopus
BBa_M11409BBa_M11409 Version 1 (Component)Promoter of pglnBP2 gene in Synechocystis sp. PCC 6803.
BBa_M11411BBa_M11411 Version 1 (Component)Type 2 promoter of lrtA gene. Gene is expressed during darkness. Potentially darkness-induced promot
BBa_K1154006BBa_K1154006 Version 1 (Component)Mating pheromone-induced IGPD and constitutive LDH expression in yeast
BBa_M11402BBa_M11402 Version 1 (Component)5' UTR and RBS of psbA2 gene in Synechocystis sp. PCC 6803
BBa_K323164BBa_K323164 Version 1 (Component)VioA and VioB enzymes fused with zinc fingers under pBAD promoter in pSB4K5
BBa_K2123114BBa_K2123114 Version 1 (Component)Stationary phase promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)
PrtDEFBBa_K258007 Version 1 (Component)Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
BBa_K2123116BBa_K2123116 Version 1 (Component)Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.