Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei-Tai Chen
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:48
sRNA targets histidine kinase 11 mRNA in S.mutans
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1510105_sequence (Version 1) |
Description
veg promoter(constitutive promoter in S.mutans+ Histidine kinase 11 sRNA+ MicC scaffold+ terminator. Constitutive promoter(veg promoter) is came from Bacillus subtilis, and MicC scaffold is came from E.coli k-12 MG1655.Other parts are synthesized by primer.This part will transcibe into sRNA, and sRNA will bind to mRNA of HK11, prevent HK11 mRNA from translating. Because histidine kinase 11 is a biofilm formation-related protein, this part is created to decrease biofilm formation.
Notes
Because we need to put our part into vector PVA838 to shuttle plasmid between E.coli and S.mutans. The restriction site before part is EcoRI and BamHI in the last. In addition, to achieve best efficiency, we also consider the spacer between promoter and coding sequence without RBS(because our final product is RNA)Source
Veg promoter+HK11 sRNA+MicC scaffold+terminator| Sequence Annotation | Location | Component / Role(s) |
| Veg promoter sRNA targets histidine kinase 11 MicC sacffold terminator | 1,36 42,65 66,144 145,157 | promoter feature/promoter feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/stop stop_codon |