BBa_K1039022
BBa_K1039022 Version 1 (Component)
PhiC31 integrase and PhiC31 RDF under lock and key control with lacI Expression Cassette

BBa_K1039021
BBa_K1039021 Version 1 (Component)
Bxb1 integrase and Recombination Directionality Factor (RDF) Under ???Lock and Key??? Control with L

BBa_I714077
BBa_I714077 Version 1 (Component)
[R0010][I714075] ([pLac][Key1][Double Terminator])

BBa_I714074
BBa_I714074 Version 1 (Component)
[R0010][J23066] ([pLac][Key3][Double Terminator])

BBa_K1216006
BBa_K1216006 Version 1 (Component)
Acetyl esterase (aes) from Escherichia Coli with TEV and poly-HIS tags

BBa_J01069
BBa_J01069 Version 1 (Component)
TEST [pBad/araC][Key1][pTet][Lock1][RFP]

BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock

BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

BBa_J01072
BBa_J01072 Version 1 (Component)
TEST [pBad/araC][Key2][pTet][Lock2][RFP]

BBa_J01070
BBa_J01070 Version 1 (Component)
TEST [pBad/araC][Key1][pTet][Lock2][RFP]

BBa_J01071
BBa_J01071 Version 1 (Component)
TEST [pBad/araC][Key2][pTet][Lock1][RFP]

BBa_I714040
BBa_I714040 Version 1 (Component)
[I714030][J23066] ([F-oriT][Key2][Double Terminator])

BBa_K1678008
BBa_K1678008 Version 1 (Component)
A biobrick shuttle plasmid working between Lactobacillus plantarum, and E.coli with erythromycin res

BBa_K1379021
BBa_K1379021 Version 1 (Component)
Key 1-B0015

BBa_J23121
BBa_J23121 Version 1 (Component)
J01003::J23007 key3 and OriT (goes with J23120)

BBa_K1379031
BBa_K1379031 Version 1 (Component)
pBad/araC-Key 1

TraJR Nonm
BBa_J01025 Version 1 (Component)
Non mobilizable TraJR, OnRFP, Key1 = [pRM TraJR][DblTerminator][OnRFP][OnKey1]

BBa_J01056
BBa_J01056 Version 1 (Component)
Non mobilizable TraJF, OnCFP, Key2 = [pBad/araC TraJF][DblTerminator][OnCFP][OnKey2]

BBa_K587010
BBa_K587010 Version 1 (Component)
Tast -> Key sequence for low concentration mechanism

BBa_K587011
BBa_K587011 Version 1 (Component)
Tawk -> Key sequence for high concentration mechanism

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.