BBa_K175034

BBa_K175034 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K175034

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Daniel Solis Escalante

Date created: 2009-09-30 11:00:00

Date modified: 2015-05-08 01:10:59

Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

Details

• Types: DnaRegion
• Roles: Project; engineered_region
• Sequences: BBa_K175034_sequence (Version 1)

Description

This biobrick has been used to test the functionality of the Lock/key for weak RBS (B0031).

Notes

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Source

This biobrick was assembled with biobricks from the registry and two new biobricks designed and constructed by TUDelft iGEM 09.

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_K175030	KWR
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012
public	BBa_R0040	p(tetR)
public	BBa_K175029	LWR
public	BBa_E0040	GFP
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_K175030	209,294	KWR
BBa_B0010	303,382	BBa_B0010
BBa_B0012	391,431	BBa_B0012
BBa_R0040	440,493	p(tetR)
BBa_K175029	502,550	LWR
BBa_E0040	557,1276	GFP
BBa_B0010	1285,1364	BBa_B0010
BBa_B0012	1373,1413	BBa_B0012

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K175034/1

Member of these Collections

• iGEM Parts Registry