Description
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Source
The parts were obtain through a extensive literature review and by search of plasmids in databases such as NCBI and AddGene.
References
DiCarlo, JE., Norville, JE., Mali, P., Rios, X., Aach, J., Church, GM., "Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.", Nucleic acids research, 2013, Apr 41(7): 4336-43, PMID:
23460208., DOI:
10.1093/nar/gkt135.
So, YS., Yang, DH., Jung, KW., Huh, WK., Bahn, YS., "[object Object]", Journal of microbiology and biotechnology, 2017, Feb 28; 27(2): 357-364, PMID:
27780958., DOI:
10.4014/jmb.1609.09036.
Cox, GM., Rude, TH., Dykstra, CC., Perfect, JR., "The actin gene from Cryptococcus neoformans: structure and phylogenetic analysis.", Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology, 1995, 33(4): 261-6, PMID:
8531025.