Cryptococcus neoformans Database in Synthetic Biology Open Language

Description

This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Source

The parts were obtain through a extensive literature review and by search of plasmids in databases such as NCBI and AddGene.

References

DiCarlo, JE., Norville, JE., Mali, P., Rios, X., Aach, J., Church, GM., "Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.", Nucleic acids research, 2013, Apr 41(7): 4336-43, PMID: 23460208, DOI: 10.1093/nar/gkt135.

Cox, GM., Rude, TH., Dykstra, CC., Perfect, JR., "The actin gene from Cryptococcus neoformans: structure and phylogenetic analysis.", Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology, 1995, 33(4): 261-6, PMID: 8531025.

Arras, SD., Chua, SM., Wizrah, MS., Faint, JA., Yap, AS., Fraser, JA., "Targeted Genome Editing via CRISPR in the Pathogen Cryptococcus neoformans.", PloS one, 2016, 11(10): e0164322, PMID: 27711143, DOI: 10.1371/journal.pone.0164322.

Wang, Y., Wei, D., Zhu, X., Pan, J., Zhang, P., Huo, L., Zhu, X., "A 'suicide' CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans.", Scientific reports, 2016, Aug 09; 631145, PMID: 27503169, DOI: 10.1038/srep31145.

Erpf, PE., Stephenson, CJ., Fraser, JA., "amdS as a dominant recyclable marker in Cryptococcus neoformans.", Fungal genetics and biology : FG & B, 2019, Oct 131103241, PMID: 31220607, DOI: 10.1016/j.fgb.2019.103241.