Cryptococcus neoformans Database in Synthetic Biology Open Language

Description

This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Source

The parts were obtain through a extensive literature review and by search of plasmids in databases such as NCBI and AddGene.

References

So, YS., Yang, DH., Jung, KW., Huh, WK., Bahn, YS., "[object Object]", Journal of microbiology and biotechnology, 2017, Feb 28; 27(2): 357-364, PMID: 27780958., DOI: 10.4014/jmb.1609.09036.

Cox, GM., Rude, TH., Dykstra, CC., Perfect, JR., "The actin gene from Cryptococcus neoformans: structure and phylogenetic analysis.", Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology, 1995, 33(4): 261-6, PMID: 8531025.

Arras, SD., Chua, SM., Wizrah, MS., Faint, JA., Yap, AS., Fraser, JA., "Targeted Genome Editing via CRISPR in the Pathogen Cryptococcus neoformans.", PloS one, 2016, 11(10): e0164322, PMID: 27711143., DOI: 10.1371/journal.pone.0164322.

Luberto, C., Martinez-Mariño, B., Taraskiewicz, D., Bolaños, B., Chitano, P., Toffaletti, DL., Cox, GM., Perfect, JR., Hannun, YA., Balish, E., Del Poeta, M., "Identification of App1 as a regulator of phagocytosis and virulence of Cryptococcus neoformans.", The Journal of clinical investigation, 2003, Oct 112(7): 1080-94, PMID: 14523045.

Varma, A., Kwon-Chung, KJ., "Characterization of the glyceraldehyde-3-phosphate dehydrogenase gene [correction of glyceraldehyde-3-phosphate gene] and the use of its promoter for heterologous expression in Cryptococcus neoformans, a human pathogen.", Gene, 1999, May 31; 232(2): 155-63, PMID: 10352226.