| Types | circular DnaRegion |
| Roles | engineered_plasmid |
| Sequences | IBMc190_seq (Version 1) |
| Sequence Annotation | Location | Component / Role(s) |
| RiboJ p15A origin of replication cat (BsmBI-) araC ECK120033736 ECK120033737 ECF20 102-193 VF2 ECK120029600 2x(GGGGS) linker 2x(GGGGS) linker tetR promoter acVHH RBS for 2nd CDS ECF20 1-101 RBS for CDS 1 BBa_B0033 BCD22 PBAD(SapI-) RFC10 Prefix (full) PBAD(SapI-) tetR acVHH L3S2P55 VR cat promoter RFC10 Suffix | 4746,4820 6217,7041 complement(3615,4274) complement(1516,2394) 5583,5635 complement(3530,3586) 5296,5571 1371,1390 3432,3521 5266,5295 3034,3063 9,97 4909,5265 4891,4899 2731,3033 4840,4847 2714,2724 4821,4908 2422,2705 1494,1515 4457,4740 98,1291 3064,3420 4381,4437 complement(5751,5770) complement(4275,4372) 5636,5656 | insulator origin_of_replication CDS CDS terminator terminator CDS primer_binding_site terminator CDS CDS promoter CDS ribosome_entry_site CDS ribosome_entry_site ribosome_entry_site biological_region promoter biological_region promoter CDS CDS terminator primer_binding_site promoter biological_region |