| Types | circular DnaRegion |
| Roles | engineered_plasmid |
| Sequences | IBMc396_seq (Version 1) |
| Sequence Annotation | Location | Component / Role(s) |
| trfA CDS (replication protein) ECK120033736 RFC10 Suffix 3x(GGGGS) linker BBa_B0030 H6 acVHH 29-1 PACE T7 RNAP 1-179 variant: L32S, E35G, A83A, K98R, L106L, Q107K, T122S, A144T pSEVA PS2 Rev PBAD(SapI-) cat RFC10 Prefix (full) pSEVA PS1 Fwd araC | complement(3615,4763) 2265,2317 2318,2338 1852,1896 1276,1290 1300,1317 1897,2253 1318,1851 complement(2447,2463) 984,1267 2638,3297 56,77 5804,5821 complement(78,956) | CDS terminator biological_region CDS ribosome_entry_site CDS CDS CDS primer_binding_site promoter CDS biological_region primer_binding_site CDS |