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IBMc397

IBMc397 Version 1

Component

Created by: Baojun Wang,Trevor Y. H. Ho

pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T

Details

Types	circular DnaRegion
Roles	engineered_plasmid
Sequences	IBMc397_seq (Version 1)

Description

pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH

Notes

Creator: Trevor Y. H. Ho
Principal Investigator: Baojun Wang
BioSafety Level: Level 1
Backbone: pSB3K3
Origin of replication: p15A
Selection Markers: Kanamycin

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
L3S3P21 RFC10 Suffix BBa_B0030 BBa_B0015 acVHH kanR p15A origin of replication L3S2P11 H6 PBAD(SapI-) T7 181+ VF2 VR RFC10 Prefix (full) araC 3x(GGGGS) linker	1273,1325 5293,5313 2597,2611 5164,5292 2639,2995 complement(5529,6344) complement(6676,6768) complement(1,732) complement(1334,1390) 2621,2638 2305,2588 3041,5155 1133,1152 complement(5447,5466) 1251,1272 complement(1399,2277) 2996,3040	terminator biological_region ribosome_entry_site terminator CDS CDS origin_of_replication terminator CDS promoter CDS primer_binding_site primer_binding_site biological_region CDS CDS

Other Properties

genbank#date	18-NOV-2020
genbank#molecule	ds-DNA
synbiohub#ownedBy	user/trevorho
synbiohub#topLevel	IBMc397/1

Member of these Collections

Intein_assisted_Bisection_Mapping

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