Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:20
TE from coliphage T7 (+/-)
| Types | DnaRegion |
| Roles | terminator Terminator |
| Sequences | BBa_B0013_sequence (Version 1) |
Description
Strong transcriptional terminator consisting of a 20 bp stem-loop that has been engineered to be bidirectional.Notes
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream.
Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Source
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4| Sequence Annotation | Location | Component / Role(s) |
| T7 TE stop C C '' polya BBa_B0013 | 14,33 40,40 37,37 10,10 1,6 34,47 1,47 | stem_loop feature/stem_loop feature/stop stop_codon feature/mutation sequence_alteration sequence_alteration feature/mutation feature/polya polyA_site feature/polya polyA_site feature/BioBrick engineered_region |