|Sequences|| BBa_B0013_sequence (Version 1)|
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Subsequently mutations were introduced to make the terminator bi-directional (i.e., AAAAAA insertion on 5' side of the stem loop). Additional mutations were introduced on the 3' side of the stem loop to increase the number of T's and eliminate any promoter -10 site that might be present to avoid initiation of transcription of whatever is downstream.
Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
|Sequence Annotation||Location||Component / Role(s)|
|stem_loop feature/stem_loop |
iGEM 2018 Cell E. coli strain NEB 10-beta
iGEM 2018 Distribution Plate 2
iGEM 2018 Distribution Plate 2 Well 2D
iGEM 2018 Part Library
iGEM 2018 Plasmid pSB1C3
iGEM 2019 Cell E. coli strain NEB 10-beta
iGEM 2019 Distribution Plate 2
iGEM 2019 Distribution Plate 2 Well 1D
iGEM 2019 Part Library
iGEM 2019 Plasmid pSB1C3
iGEM Parts Registry