Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Heather Keller
Date created: 2006-09-27 11:00:00
Date modified: 2015-08-31 04:07:21
T7 RBS 0.5
| Types | DnaRegion |
| Roles | ribosome_entry_site RBS |
| Sequences | BBa_B2003_sequence (Version 1) |
Description
This is the -20 to -1 region of T7 gp0.5, which contains the native RBS region for this gene. The physical instantiation of this part does not exist. This part is not compatible with Standard Biobricks assembly methods, as the scar created during assembly would interrupt the spacing between the RBS and the start codon of the CDS. Individuals wishing to use this RBS sequence in their assembles should refer to part B2103.Notes
The -20 to -1 region was chosen to be representative of the RBS region of the T7 genes for the following reasons:1) The Shine-Delgarno region is contained within this defined region for all T7 genes.
2) Current literature suggests that when bound to the Translation Start Site (0), the Ribosome covers positions -20 to +13 of the gene.