Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Debra Lin, Srini Devadas, David Gray, Ronny Krashinsky, and Chris Zheng Liu, Boopers
Date created: 2004-01-26 12:00:00
Date modified: 2015-08-31 04:07:23
autoinducer synthetase for N-butyryl-HSL (BHL) and HHL
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_C0070_sequence (Version 1) |
Description
Autoinducer synthesis protein that produces N-butyryl-HSL which binds to RhlR, obtained from Pseudomonas aeruginosa.Notes
Editing Check (1/28/04)(0) BB sites cleaned
(1) ATG
(2) TAATAA
(3) Blast checks out (i.e., it's RhlI)
(4) Codon changes OK (AA and tRNA usage)
(5) Chassis might have conflict (w/ native autoinducer system?)
(6) ssrA tag added
(7) barcode added
(8) Codon optimized for expression in enteric bacteria
2 silent point mutations have been included into the sequence at base 138 from A to G and at base 576 from G to C to remove internal EcoRI and PstI sites. Mutations were chosen to yield the same amino acid and codons of similar frequency in E. coli.