mnt (s)

BBa_C0072 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_C0072
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: crackdots
Date created: 2004-01-28 12:00:00
Date modified: 2015-08-31 04:07:23

mnt repressor (strong) from Salmonella phage P22 (+LVA)



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_C0072_sequence (Version 1)

    Description

    -- No description --

    Notes

    wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12.



    sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99).



    Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor

    Kendall, KL; Sauer, RT

    The Journal of Biological Chemistry, 264(23):13706-13710, 1989

    Source

    enterobacteriophage p22

    Sequence Annotation Location Component / Role(s)
    Help:Barcodes
    mnt
    T
    LVA
    		289,313
    1,249
    95,97
    250,282
    		feature/barcode engineered_tag
    CDS feature/cds
    feature/s_mutation point_mutation
    feature/tag tag

    igem#experience
    None
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_C0072/1
     

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