mnt (s)

BBa_C0072 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_C0072
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: crackdots
Date created: 2004-01-28 12:00:00
Date modified: 2015-08-31 04:07:23

mnt repressor (strong) from Salmonella phage P22 (+LVA)



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_C0072_sequence (Version 1)

Description

-- No description --

Notes

wild type dimeric repressor from enterobacteriophage p22 with dissociation constant of 2.5*10^-12.



sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99).



Identification of Functionally Important Residues in the DNA Binding Region of the MNT Repressor

Kendall, KL; Sauer, RT

The Journal of Biological Chemistry, 264(23):13706-13710, 1989

Source

enterobacteriophage p22

Sequence Annotation Location Component / Role(s)
Help:Barcodes
mnt
T
LVA
289,313
1,249
95,97
250,282
feature/barcode engineered_tag
CDS feature/cds
feature/s_mutation point_mutation
feature/tag tag
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_C0072/1