Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: crackdots
Date created: 2004-01-27 12:00:00
Date modified: 2015-08-31 04:07:23
mnt repressor (weak) from Salmonella phage P22 (+LVA)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_C0073_sequence (Version 1) |
Description
-- No description --Notes
dimeric repressor from enterobacteriophage p22 with dissociation constant of 3*10^-8.sequence was translated with the E.Coli K12 codon usage table, and nucleotides 95-97 was changed from AAT to AAC to avoid the cut site GAATTC (94-99).
the mutation R10->K10 (ctg->aaa) decreases the binding affinity of the mnt protein to its operator by approximately 12,000 fold (Kd: 2.5e-12 to 3e-8)
Source
enterobacteriophage p22| Sequence Annotation | Location | Component / Role(s) |
| Help:Barcodes mnt LVA Arg->Lys (ctg) T | 289,313 1,249 250,282 31,33 95,97 | feature/barcode engineered_tag CDS feature/cds feature/tag tag sequence_alteration feature/mutation point_mutation feature/s_mutation |