Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Drew Endy
Date created: 2004-01-29 12:00:00
Date modified: 2015-08-31 04:07:25
Zif23-GCN4 engineered repressor (+LVA, C2000 codon-optimized for E.coli)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_C2001_sequence (Version 1) |
Description
Variant of Zif23-GCN4 (see BBa_C2000). CDS has been optimized (via VNTI) for expression in enteric bacteria. ssrA tag (LVA) added.Notes
This protein should form a dimer and bind a specific operator site, thus repressing transcription.Source
Protein Engineering Gods from the Pabo Lab| Sequence Annotation | Location | Component / Role(s) |
| Help:Barcodes Finger 2 Finger 3 linker GCN4: leucine zipper LVA 2 Recognition site Recognition site | 301,325 4,90 91,150 151,165 166,261 262,294 295,300 43,63 127,147 | feature/barcode engineered_tag feature/cds CDS feature/cds CDS sequence_feature feature/misc CDS feature/cds tag feature/tag stop_codon feature/stop feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site |