Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2006-10-20 11:00:00
Date modified: 2015-08-31 04:07:25
Homodimeric zinc finger
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_C2004_sequence (Version 1) |
Description
This is protein based on Scot Wolfe's Zif268-GCN4 homodimeric zinc finger protein. It is a fusion of fingers 2 and 3 of Zif268 (mouse) and the leucine zipper domain of GCN4 (yeast). Scot optimized the linker between the zinc finger domain and the leucine zipper domain to increase affinity of the homodimer for the 12bp operator. It is codon optimized for E. coli.Notes
It is codon optimized for E. coli.Source
It was derived from BBa_C2002.| Sequence Annotation | Location | Component / Role(s) |
| Finger 2 of Zif268 start codon Zif268.GCN4(-2) codon optimized hexaHis tag Finger 3 of Zif268 domain linker GCN4 homodimerization domain double stop codon | 4,90 1,3 1,288 265,282 91,150 151,177 178,264 283,288 | non_covalent_binding_site feature/binding start_codon feature/start CDS feature/cds feature/tag tag non_covalent_binding_site feature/binding sequence_feature feature/misc feature/binding non_covalent_binding_site stop_codon feature/stop |