Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2007-08-21 11:00:00
Date modified: 2015-08-31 04:07:25
MBP.p53ZF F2F3.GCN4 homodimeric repressor
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_C2008_sequence (Version 1) |
Description
Maltose binding protein fused to fingers 2 and 3 of p53ZF (a mutated Zif268 designed to bind to a different operator) fused to the GCN4 leucine zipper.Notes
Two silent mutations were introduced to remove an internal NotI site and a rare arginine codon.Source
This coding region was rationally designed based on BBa_C2006 and p53ZF.| Sequence Annotation | Location | Component / Role(s) |
| malE maltose binding protein (MBP) start codon MBP.p53ZF F2F3.GCN4 GCN4 leucine zipper double TAA stop codon p53ZF F2F3 zinc finger DNA binding domain | 1,1101 1,3 1,1428 1327,1422 1423,1428 1168,1311 | CDS feature/protein feature/start start_codon feature/cds CDS CDS feature/protein stop_codon feature/stop CDS feature/protein |