Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Austin Che and Reshma Shetty
Date created: 2007-07-22 11:00:00
Date modified: 2015-08-31 04:07:25
GFP.lacZalpha fusion
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_E0050_sequence (Version 1) |
Description
This part is a fusion protein between GFP (BBa_E0040) and the N-terminal alpha fragment of lacZ (which encodes β-galactosidase. It is a reporter which can be measured via fluorescence from GFP or through a Miller assay of β-galactosidase.Notes
I have included the complete GFP sequence. The boundaries of the lacZ alpha fragment are the first 60 codons of lacZ. This is what is defined as the alpha peptide in "The lac operon" by Benno Muller-Hill (see page 76).Source
The GFP sequence comes from (BBa_E0040) and the N-terminal alpha fragment of lacZ comes directly from lacZ.| Sequence Annotation | Location | Component / Role(s) |
| BBa_E0040 (GFP) lacZ alpha peptide double stop codon | 1,714 715,894 895,900 | CDS feature/cds feature/cds CDS feature/stop stop_codon |