Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:29
CI(1) "
KISS"
asRNA without promoter
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_I1011_sequence (Version 1) |
Description
Region which serves as basis for transcription of asRNA that binds to and inhibitsNotes
References (unparsed) here:Coleman, J., et al. Nature. (1985) 315, 601-3.
Pestka, S., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 7525-28.
References (unparsed) here:
Coleman, J., et al. Nature. (1985) 315, 601-3.
Pestka, S., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 7525-28.
Complementary to beginning of
Anti-senseThe success of this system clearly rests on the ability to effectively and specifically target mRNA transcripts for degradation using anti-sense RNA. While many papers, articles, and books have been written on the subject, there are no consensus anti-sense building strategies presented. We thus chose to implement three different types of antisense inhibition: KISS, micRNA, and IS10. In the description that follows, the following nomenclature will be used:
target- the mRNA transcript that we wish to inhibit.
anti-sense- the anti-sense molecule which will bind and inhibit target.
KISS (Keep it SImple, Silly)
The simplest of the three methods, this type relies on a single-stranded linear 103 bp anti-sense that is specific to the target of interest. In addition, the first 76 base pairs of the cI region of BBa_I1010 have been codon-modified to give a different sequence that codes for the same cI protein (See BBa_I1030 and I1040).
BBa_I1011 contains the reverse complement of the RBS, start codon, and 76 bp region for BBa_I1010. Thus, if both BBa_I1010 and BBa_I1011 are transcribed, the transcripts will bind to each other and BBa_I1010 will not be translated.
Note that BBa_I1010 already contains a regulatory region, RBS, and coding region (a terminator must be added), while BBa_I1011 does not - thus, when using this component, the appropriate regulatory region, RBS, and terminator must be added to this part.
Incompatible with systems containing
Compatible with
Source
Custom design.| Sequence Annotation | Location | Component / Role(s) |
| Reverse Complement to cI mRNA Reverse Complement RBS reverse complement to cI cds start added codon (Cys) BBa_I1011 | 1,103 81,86 1,73 74,76 71,73 1,103 | sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/start start_codon feature/mutation sequence_alteration feature/BioBrick engineered_region |