Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Sutton, Neelaksh Varshney, Daniel Shen, Grace Kenney
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:29
Custom cI(2) Protein w/LacO-1 Promoter
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_I1020_sequence (Version 1) |
Description
Coding sequence for modified cI protein with LacO-1 promoter for antisense IS10 mRNA regulation.Notes
Can be used with all parts of the BBa_I system. It is inhibited by BBa_I1023. Incompatible with systems containing cI (wild type)
Source
Modified from BioBricks| Sequence Annotation | Location | Component / Role(s) |
| stem_loop rbs start cI lambda -35 -10 Complementary to Antisense mRNA I1023 SsrA 2 LacO-1 region LacO-1 start HH117a mutation specific to I1020 and I1023 C added Homology to I1023 BBa_I1020 | 56,85 73,78 83,85 83,835 21,26 44,49 56,90 797,835 830,835 1,20 27,43 56,56 58,58 86,88 89,90 1,835 | stem_loop feature/stem_loop ribosome_entry_site feature/rbs start_codon feature/start CDS feature/cds promoter feature/promoter promoter feature/promoter sequence_feature feature/misc tag feature/tag feature/stop stop_codon non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/start start_codon feature/mutation sequence_alteration sequence_alteration feature/mutation sequence_feature feature/misc engineered_region feature/BioBrick |