BBa_I1021

BBa_I1021 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I1021

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: June Rhee, Connie Tao, Ty Thomson, Louis Waldman

Date created: 2003-01-31 12:00:00

Date modified: 2015-08-31 04:07:29

Antisense RNA w/BBa_I1020 Interference (KISS)

Details

• Types: DnaRegion
• Roles: mature_transcript_region; RNA
• Sequences: BBa_I1021_sequence (Version 1)

Description

Sequence for antisense RNA that interferes with BBa_I1020 mRNA using the KISS scheme.

Notes

BBa_I1021 antisense RNA is designed to interfere with BBa_I1020 mRNA with no expected secondary structure interactions (dubbed the KISS scheme for Keep is Simple Samantha). The sequence is designed to interfere exclusively with BBa_I1020, and act independently of BBa_I1010 and BBa_I1011. These parts are designed to use codon space to code for the same protein (BBa_I1010 and BBa_1020 code for lambda cI) while allowing for independent anti-sense regulation of the BBa_I1010 and BBa_1020 transcripts via BBa_I1011 and BBa_1021

Source

Anti-sense secondary structure adapted from: Mizuno, T., Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
suffix	101,104	feature/misc, sequence_feature
start	71,73	feature/start, start_codon
cI mRNA reverse complement	1,100	sequence_feature, feature/misc
cI CDS Reverse Complement	1,73	feature/misc, sequence_feature
reverse complement	78,83	feature/rbs, ribosome_entry_site
BBa_I1021	1,104	feature/BioBrick, engineered_region

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I1021/1

Member of these Collections

• //classic/rna/uncategorized
• iGEM Parts Registry