BBa_I1023

BBa_I1023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I1023
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grace Kenney, Neelaksh Varshney, Samantha Sutton , Daniel Shen
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:29

CI(2) IS10 asRNA



Types
DnaRegion

Roles
RNA

mature_transcript_region

Sequences BBa_I1023_sequence (Version 1)

Description

Region which serves as basis for transcription of asRNA that binds to and inhibits BBa_I1020's mRNA transcript. Part of the XOR gate comprised of BBa_I1010 and BBa_I1020, their corresponding asRNA coding sequences (BBa_I1013 and BBa_I1023).

Notes

References (unparsed) here:

A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000

Case, C., Roels, S., Jense, P., Lee, J., Kleckner, N. and Simons, R. (1989). The unusual stability of the IS10 anti-sense RNA is critical for its function and is determined by the structure of its stem-domain. EMBO 8(13): 4297-4305.

Jain, C. (1995). IS10 Antisense Control in Vivo is Affected by Mutations Throughout the Region of Complementarity Between the Interacting RNAs. J. Mol. Biol. 246:585-594.

Kittle, J.D., Simons, R.W., Lee, J., and Kleckner, N. (1989). Insertion Sequence IS10 Anti-sense Pairing Initiates by an Interaction Between the 5' End of the Target RNA and a Loop in the Anti-sense RNA. J. Mol. Biol. 210:561-572.

Jain, C. (1997). Models for Pairing of IS10 Encoded Antisense RNAs in vivo. J. theor. Biol. 186: 431-439.

Lutz, R., and Bujard, H. (1997). Independent and tight regulation of transcriptional units in E. coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research 25(6): 1203-1210.

Ma, C., and Simons, R. (1990). The IS10 antisense RNA blocks ribosome binding at the transposase translation initiation site. EMBO 9(4):1267-1274.

E. coli codon usage table at http://bioinfo.weizmann.ac.il:3456/kegg/codon_table/codon_eco.html.


References (unparsed) here:

A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000

Case, C., Roels, S., Jense, P., Lee, J., Kleckner, N. and Simons, R. (1989). The unusual stability of the IS10 anti-sense RNA is critical for its function and is determined by the structure of its stem-domain. EMBO 8(13): 4297-4305.

Jain, C. (1995). IS10 Antisense Control in Vivo is Affected by Mutations Throughout the Region of Complementarity Between the Interacting RNAs. J. Mol. Biol. 246:585-594.

Kittle, J.D., Simons, R.W., Lee, J., and Kleckner, N. (1989). Insertion Sequence IS10 Anti-sense Pairing Initiates by an Interaction Between the 5' End of the Target RNA and a Loop in the Anti-sense RNA. J. Mol. Biol. 210:561-572.

Jain, C. (1997). Models for Pairing of IS10 Encoded Antisense RNAs in vivo. J. theor. Biol. 186: 431-439.

Lutz, R., and Bujard, H. (1997). Independent and tight regulation of transcriptional units in E. coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research 25(6): 1203-1210.

Ma, C., and Simons, R. (1990). The IS10 antisense RNA blocks ribosome binding at the transposase translation initiation site. EMBO 9(4):1267-1274.

E. coli codon usage table at http://bioinfo.weizmann.ac.il:3456/kegg/codon_table/codon_eco.html.


Complementary to beginning of BBa_I1020 transcript covering 5' region, RBS, start codon, and 5 bp into coding sequence. Secondary structure designed for the IS10 anti-sense mRNA mechanism (See below).



Source

see referencers

Sequence Annotation Location Component / Role(s)
stem_loop
RBS Reverse Complement
start
stem_loop
HH117a mutation specific to I1020 and I1023
Extra Met for stem homology
First 2 bp of cI I1020
start
TetR 1
-35
TetR 2
-10
TetR
BBa_I1023
78,98
67,72
60,62
60,116
87,87
57,59
55,56
55,55
1,19
20,25
26,44
43,48
1,54
1,169
feature/stem_loop stem_loop
feature/misc sequence_feature
feature/start start_codon
stem_loop feature/stem_loop
sequence_alteration feature/mutation
sequence_alteration feature/mutation
feature/misc sequence_feature
feature/start start_codon
feature/binding non_covalent_binding_site
feature/promoter promoter
feature/binding non_covalent_binding_site
promoter feature/promoter
promoter feature/promoter
engineered_region feature/BioBrick
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I1023/1