BBa_I1030

BBa_I1030 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I1030

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton

Date created: 2003-01-31 12:00:00

Date modified: 2015-08-31 04:07:29

RNA Regulation Target ( Promoter driven by C0040 and cI mod #1 CDS)

Details

• Types: DnaRegion
• Roles: RNA; mature_transcript_region
• Sequences: BBa_I1030_sequence (Version 1)

Description

Coding region for the tetR promoter (BBa_C0040) with antisense binding region for BBa_I1031 and BBa_I1032, and a codon modified cI(1) protein with an LVA degradation tail.

Notes

BBa_I1030 Custom cI Protein with TetR Promoter is based on BioBricks BBa_C0050 and BBa_R0040. It is designed such that BBa_I1031 and BBa_I1032can be used to interfere with the BBa_1030 transcript by anti-sense mRNA binding, in the KISS or micRNA method shown below. BBa_I1030 and BBa_I1031 or BBa_I1032 can act indepently of related biobrick parts BBa_I1041, BBa_I1042, BBa_I1020, BBa_BBa_I1023 and BBa_1013. [KEIL96]

Incompatible with systems containing IPTG, or cI.

May be some cross talk with systems containing BBa_I1010. Compatible with all other BBa_line I-line parts.

Proper operation requires AMP/CAP complex, which should normally be present in the cell.

Source

see references

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cI	91,840	CDS, feature/cds
start	91,93	feature/start, start_codon
modified cI1 cds	94,163	CDS, feature/cds
TetR 1	1,19	non_covalent_binding_site, feature/binding
-35	20,25	feature/promoter, promoter
TetR 2	26,44	feature/binding, non_covalent_binding_site
-10	43,48	promoter, feature/promoter
TetR	1,54	promoter, feature/promoter
SsrA	802,840	feature/tag, tag
2	835,840	feature/stop, stop_codon
intentional junk dna	55,74	feature/misc, sequence_feature
start	55,55	feature/start, start_codon
rbs	77,82	ribosome_entry_site, feature/rbs
intentional junk dna	85,88	sequence_feature, feature/misc
antisense RNA pairing	55,162	sequence_feature, feature/misc
BBa_I1030	1,840	engineered_region, feature/BioBrick

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I1030/1

Member of these Collections

• //classic/rna/uncategorized
• iGEM Parts Registry