BBa_I1040

BBa_I1040 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I1040

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton

Date created: 2003-01-31 12:00:00

Date modified: 2015-08-31 04:07:29

RNA Regulation Target ( Promoter driven by C0010 and cI(2) CDS)

Details

• Types: DnaRegion
• Roles: mature_transcript_region; RNA
• Sequences: BBa_I1040_sequence (Version 1)

Description

Coding region for the LacO-1 promoter with antisense binding region for BBa_I1041 and BBa_I1042, and a codon modified cI(1) protein with an LVA degradation tailCoding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator (BBa_R0010) and hybrid Pl-lac01 regulator as is used in parts BBa_I1020, BBa_I1023, BBa_I1031, BBa_I1032, BBa_I1040, BBa_I1041, BBa_I1042, BBa_I1060, BBa_I1063, BBa_I1020. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, thus allowing transcription. This is the basis of an "Implies" gate.

Notes

BBa_I1040 Custom cI Protein with LacO-1 Promoter is based on BioBricks BBa_C0050. It is designed such that BBa_I1041 and BBa_I1042 can be used to interfere with the BBa_1040 transcript by anti-sense mRNA binding, in the KISS or micRNA method shown below. BBa_I1040 and BBa_I1041 or BBa_I1042 can act indepently of related biobrick parts BBa_I1031, BBa_I1032, BBa_I1020, and BBa_1013. [KEIL96]

Incompatible with systems containing IPTG, lactose, lacI, or cI.

May be some cross talk with systems containing BBa_I1020 or BBa_I1060. Compatible with all other BBa_line I-line parts.

Proper operation requires AMP/CAP complex, which should normally be present in the cell.

Source

see references

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	92,94	start_codon, feature/start
cI lambda	92,841	feature/cds, CDS
-35	21,26	promoter, feature/promoter
-10	44,49	promoter, feature/promoter
SsrA	803,841	feature/tag, tag
2	836,841	feature/stop, stop_codon
LacO-1 region	1,20	feature/binding, non_covalent_binding_site
LacO-1	27,43	feature/binding, non_covalent_binding_site
start	56,56	start_codon, feature/start
intentional junk dna	56,75	feature/misc, sequence_feature
RBS	78,83	ribosome_entry_site, feature/rbs
intentional junk dna	86,89	sequence_feature, feature/misc
antisense RNA pairing	56,163	feature/misc, sequence_feature
BBa_I1040	1,841	feature/BioBrick, engineered_region

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I1040/1

Member of these Collections

• //classic/rna/uncategorized
• iGEM Parts Registry