BBa_I1060

BBa_I1060 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I1060
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:30

Custom cI(2) Protein w/LacO-1 Promoter (Used for Anti-sense inhibition)



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_I1060_sequence (Version 1)

Description

Coding sequence for modified cI protein with LacO-1 promoter for antisense IS10 mRNA regulation.



Notes

BBa_I1060 Custom cI Protein with LacO-1 Promoter is based on BioBricks BBa_C0050 and BBa_R0011. It is designed such that BBa_I1063 can be used to interfere with the BBa_1060 transcript by anti-sense mRNA binding, in the IS10 method shown below. BBa_I1060 and BBa_I1063 can act indepently of related biobrick parts BBa_1010, BBa_1011, BBa_1012, BBa_I1030, BBa_I1031, BBa_I1032, BBa_I1041, BBa_I1042, BBa_I1020, BBa_I1023 and BBa_1013.

Can be used with all parts of the BBa_I system. It is inhibited by BBa_I1063. Incompatible with systems containing cI (wild type)

Source

Modified from BioBricks BBa_R0011, and BBa_C0051.

Sequence Annotation Location Component / Role(s)
stem_loop
rbs
start
cI lambda
-35
-10
Complemenatry to Antisense mRNA I1063
SsrA
2
LacO-1 region
LacO-1
start
HH117a swap
C added
Binds BBa_I1063
swap
swap
swap
swap
BBa_I1060
56,85
73,78
83,85
83,835
21,26
44,49
56,158
797,835
830,835
1,20
27,43
56,56
58,58
86,88
89,90
72,72
79,79
81,81
82,82
1,835
feature/stem_loop stem_loop
ribosome_entry_site feature/rbs
feature/start start_codon
CDS feature/cds
feature/promoter promoter
promoter feature/promoter
sequence_feature feature/misc
feature/tag tag
stop_codon feature/stop
feature/binding non_covalent_binding_site
feature/binding non_covalent_binding_site
feature/start start_codon
sequence_feature feature/misc
sequence_alteration feature/mutation
feature/binding non_covalent_binding_site
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
feature/misc sequence_feature
engineered_region feature/BioBrick
igem#sampleStatus
It's complicated
igem#status
Planning
 
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user/james
 
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user/myers
 
synbiohub#topLevel
BBa_I1060/1