BBa_I1063

BBa_I1063 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I1063
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:30

CI(2) IS10 asRNA



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_I1063_sequence (Version 1)

Description

Region which serves as basis for transcription of asRNA that binds to and inhibits BBa_I1060 mRNA transcript. Part of the XOR gate comprised of BBa_I1010 and BBa_I1060, their corresponding asRNA coding sequences (BBa_I1013 and BBa_I1063).

Notes

Complementary to beginning of BBa_I1060 transcript covering 5' region, RBS, start codon, and 5 bp into coding sequence. Secondary structure designed for the IS10 anti-sense mRNA mechanism (See below).

Can be used with all parts of the BBa_I system. It inhibits BBa_I1060. Incompatible with systems containing cI (wild type)

Source

see referencers

Sequence Annotation Location Component / Role(s)
stem_loop
RBS Reverse Complement
start
stem_loop
C added
Binds first 2 bf of I1060, I1020
swap
swap
swap
swap
HH117a swap
swap
swap
swap
swap
start
TetR 1
-35
TetR 2
-10
TetR
BBa_I1063
78,98
67,72
60,62
60,116
57,59
55,56
63,63
64,64
66,66
73,73
87,87
103,103
110,110
112,112
113,113
55,55
1,19
20,25
26,44
43,48
1,54
1,169
stem_loop feature/stem_loop
feature/misc sequence_feature
feature/start start_codon
stem_loop feature/stem_loop
sequence_alteration feature/mutation
non_covalent_binding_site feature/binding
feature/misc sequence_feature
sequence_feature feature/misc
feature/misc sequence_feature
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
feature/misc sequence_feature
sequence_feature feature/misc
sequence_feature feature/misc
start_codon feature/start
non_covalent_binding_site feature/binding
promoter feature/promoter
non_covalent_binding_site feature/binding
feature/promoter promoter
promoter feature/promoter
feature/BioBrick engineered_region
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I1063/1