Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: bu
Date created: 2004-09-07 11:00:00
Date modified: 2015-08-31 04:07:30
HK022 half bit
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_I11062_sequence (Version 1) |
Description
This half bit contains the integrase/excisionase for HK022 and attP/attB rev comp for P21. The attachment sites flank a unidirectional terminator. This half bit is designed to work with the P21 half bit (BBa_I11063)| Sequence Annotation | Location | Component / Role(s) |
| spacer KpnI spacer RBS spacer Integrase HK022+LVA (BBa_I11040) LVA stop spacer AclI spacer P21 attP attachment site (BBa_I11053) spacer PvuI spacer B0025 reverse terminator spacer XmaI spacer P21 attB (I11053) spacer BmtI RBS spacer cI HK022 AAV stop spacer NotI spacer | 1,3 4,9 10,12 13,18 19,23 24,1094 1095,1127 1128,1133 1134,1136 1137,1142 1143,1145 1146,1563 1564,1566 1567,1572 1573,1575 1576,1705 1706,1708 1709,1714 1715,1717 1718,1736 1737,1739 1740,1745 1746,1751 1752,1756 1757,1972 1973,2005 2006,2011 2012,2014 2015,2022 2021,2022 | feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/rbs ribosome_entry_site feature/misc sequence_feature CDS feature/cds feature/tag tag feature/stop stop_codon feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc non_covalent_binding_site feature/binding sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/stem_loop stem_loop feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/rbs ribosome_entry_site sequence_feature feature/misc CDS feature/cds feature/tag tag feature/stop stop_codon sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc |