Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hans
Date created: 2004-07-21 11:00:00
Date modified: 2015-08-31 04:07:31
Test Construct of BBa_I12007 promoter
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_I12019_sequence (Version 1) |
Description
This will yield YFP upon addition of IPTG if the BBa_I12007 promoter is working (provided there is no cII protein from phage p22).Notes
The test construct was built using BioBrick parts that were available and working. The premise behind this test construct is that the absence of p22 cII will yield a constant promotion of LacI, which will inhibit the expression of lambda cI. However, once IPTG is added, the IPTG will bind to the LacI, hence freeing the promoters of the QPI (Quad Part Inverter) and promoting production of lambda cI. Lambda cI will bind to the operators of BBa_I12007, thus promoting production of Yellow Fluorescent Protein| Access | Instance | Definition |
| public public public | BBa_I12021 BBa_I12007 BBa_E0430 | BBa_I12021 Prm + BBa_E0430 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I12021 BBa_I12007 BBa_E0430 | 1,2371 2380,2461 2470,3347 | BBa_I12021 Prm + BBa_E0430 |