BBa_I12022

BBa_I12022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I12022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ryhsiao
Date created: 2004-07-25 11:00:00
Date modified: 2015-08-31 04:07:31

Inducible 434 cI Repressor Generator (Controlled by IPTG and LacI)



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Generator

    Sequences BBa_I12022_sequence (Version 1)

    Description

    This part will produce 434 cI Repressor when IPTG is introduced into the system. The system must also be free of p22 cII for 434 cI repressor to be expressed.

    Notes

    The absence of p22 cII (constitutive promoter) will yield a constant promotion of LacI, which inhibits the expression of lambda cI. Once IPTG is introduced into the system, it will bind to the LacI, resulting in expression/promotion of 434 cI repressor via the QPI (Quad Part Inverter). Hence, a switch controlled by IPTG is created.

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I12022/1
     

    Attachments

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