Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ryhsiao
Date created: 2004-08-05 11:00:00
Date modified: 2015-08-31 04:07:31
Modified lamdba Prm promoter (repressed by 434 cI with cooperativity) RBS+
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_I12034_sequence (Version 1) |
Description
Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI) with both activation and repression taking advantage of cooperativity.Notes
The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434. To allow 434 cI to take advantage of cooperativity and to maintain spacing, the -10 site was left undisturbed, but immediately following, the space was replaced with the OR-2 site of 434 cI. Thus, if 434 cI is present in the system, it will bind to both the OR-1 and OR-2 sites of 434, preventing transcription. However, if there is no 434 cI present, then a portion of the OR-2 site will be included in the mRNA, which should not result in any problems.Source
Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40.| Sequence Annotation | Location | Component / Role(s) |
| OR1 lambda -35 OR2 lambda OR1 434 -10 OR2 434 BBa_B0034 (Elowitz) conserved | 9,25 48,53 33,49 56,69 71,76 77,91 92,103 96,101 | feature/binding non_covalent_binding_site feature/promoter promoter non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding feature/promoter promoter feature/binding non_covalent_binding_site feature/rbs ribosome_entry_site feature/conserved conserved_region |