Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Hans
Date created: 2004-08-17 11:00:00
Date modified: 2015-08-31 04:07:31
Modified lamdba Prm promoter (cooperative repression by 434 cI)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_I12036_sequence (Version 1) |
Description
Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operators are used for both activation and repression to enhance cooperativity.Notes
The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage.Source
Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40.| Sequence Annotation | Location | Component / Role(s) |
| OR1 lambda OR2 lambda OR1 434 OR2 434 -35 -10 start | 9,25 33,49 56,69 78,91 48,53 71,76 83,83 | non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site non_covalent_binding_site feature/binding promoter feature/promoter promoter feature/promoter feature/start start_codon |