Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: jkm
Date created: 2005-05-24 11:00:00
Date modified: 2015-08-31 04:07:34
Removes BioBrick End
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_I13450_sequence (Version 1) |
Description
Contains both an early MfeI site and a late NsiI site. Cutting with Mfe and Spe and ligation to a BB part cut EX will form two mixed sites, neither of which can be recut. Cutting with Xba and Nsi and ligation to a BB part cut SP will again form two mixed sites, neither of which can be recut.Notes
Should be grown in pSB1A2, as Nsi cuts both pSB2K3 and pSB1AK3 and Mfe cuts pSB4A3.70 bp from Xba to Nsi or Mfe to Spe, the minimum for gel extraction. The middle is largely noise, to raise the size of the part.